Office: 10 Schweitzer Hall
117 Schweitzer Hall
University of Missouri
Columbia, MO 65211
Phone: (573) 882-7648
Fax: (573) 882-5635
Lab: (573) 882-7300
|BS||Illinois State University||Normal, Ill.||Biology|
|MS||University of Illinois||Urbana, Ill.||Biology|
|PhD||University of Illinois||Urbana, Ill.||Biology|
Our current research is focused on understanding how specific domains in ARF and Aux/IAA proteins function in auxin-regulated transcription.
Auxin mediates a wide variety of plant growth and developmental processes, including organ patterning, lateral root initiation, stem and root elongation, vascular tissue formation and differentiation, apical dominance, and tropisms. Most of these processes are likely initiated by specific patterns of gene expression in response to localized changes in auxin concentration. Genes have been identified that are rapidly up-regulated or down-regulated with cell/tissue/organ specificity in response to auxin. Early auxin genes respond within minutes after auxin stimulation and do not require protein synthesis for a response. Aux/IAAs, GH3s, and SAURs are examples of early auxin response genes. Analysis of early auxin gene promoters has allowed us to identify cis-elements [i.e., Auxin Response Elements or AuxREs with the sequence TGTCTC] that confer auxin responsiveness and trans-factors [i.e., Auxin Response Factors or ARFs] that bind with specificity to AuxREs.
ARF genes and proteins. There are 22 genes in Arabidopsis that are predicted to encode ARF proteins [i.e., referred to as ARF1 to ARF22]. ARF proteins range in size from about 70 to 130 kDa and contain conserved N-terminal DNA-binding domains [DBDs] and C-terminal dimerization domains [CTDs], with the exception of ARF3 and ARF17, which lack a canonical CTD. ARFs also contain nonconserved regions, just C-terminal to the DBD, which function as activation [AD] or repression [RD] domains. ARF DBDs are related over a stretch of about 120 amino acids to a conserved B3 domain found in the DBDs of a variety of other plant transcription factors. ARF dimerization domains are composed of motifs III and IV and are related in amino acid sequence to motifs III and IV in the dimerization domains of Aux/IAA proteins. These motifs facilitate homotypic and heterotypic interactions among ARF and Aux/IAA proteins. The AD/RDs of ARFs have biased amino acid sequences, and those that are Q-rich function as ADs [i.e., ARF5, -6, -7, -8, -19] in protoplast transfection assays. The remaining ARFs function as repressors, at least in transfected plant protoplasts. Mutations in ARF genes have provided insight into auxin-dependent processes that ARF transcription factors regulate.
Aux/IAA genes and proteins. Arabidopsis contains 29 Aux/IAA genes and many of these are early auxin genes. Some are induced within 5-10 minutes following auxin treatment. Aux/IAA genes encode proteins of 20-35 kDa that, in general, share four motifs [i.e., I, II, III, and IV] of amino acid sequence similarity. Several Aux/IAA proteins have been shown to have short half-lives [i.e., 5-10 minutes] and are localized to the nucleus. While Aux/IAA proteins do not appear to bind DNA in a selective manner, they, nevertheless, function as active repressors by dimerizing with ARF activators on auxin-responsive genes. A number of dominant or semidominant auxin response mutants have been identified that have amino acid substitutions within motif II of Aux/IAA proteins. These mutations result in stabilization of Aux/IAA proteins, and mutant plants show alterations in auxin sensitivity and responses, including increased resistance to exogenous auxin, abnormalilties in lateral root and root hair formation, and defects in root and hypocotyl gravitropism. In some cases, loss-of-function arf mutants and gain-of-function aux/iaa mutants have similar phenotypes, suggesting that these ARF and Aux/IAA proteins may function as partners in regulating auxin response genes in specific cells/tissues or at specific stages of development.
Interplay of ARFs and Aux/IAA proteins in regulating auxin response genes. ARF and Aux/IAA proteins are thought to function together in regulating expression of early auxin genes. ARF activators bind with specificity to TGTCTC AuxREs and dimerize with Aux/IAA repressors when auxin concentrations are low, resulting in dominant, active repression. When auxin concentrations are elevated, Aux/IAA repressors become increasingly unstable, resulting in their decreased abundance and release from the ARF activators. This in turn leads to derepression/activation of the auxin response genes. Potentiation of activation occurs when closely related ARF activators are recruited to the AuxRE via CTD dimerization. Our current research is focused on understanding how specific domains in ARF and Aux/IAA proteins function in auxin-regulated transcription.
Lavenus J, Goh T, Guyomarc'h S, Hill K, Lucas M, Voß U, Kenobi K, Wilson MH, Farcot E, Hagen G, Guilfoyle TJ, Fukaki H, Laplaze L, Bennett MJ. 2015. Inference of the Arabidopsis lateral root gene regulatory network suggests a bifurcation mechanism that defines primordia flanking and central zones. Plant Cell. 2015 May;27(5):1368-88. Epub 2015 May 5.
Guilfoyle TJ. 2015. The PB1 domain in auxin response factor and Aux/IAA proteins: a versatile protein interaction module in the auxin response. Plant Cell. 2015 Jan;27(1):33-43. doi: 10.1105/tpc.114.132753. Epub 2015 Jan 20. Review.
Nanao MH, Vinos-Poyo T, Brunoud G, Thévenon E, Mazzoleni M, Mast D, Lainé S, Wang S, Hagen G, Li H, Guilfoyle TJ, Parcy F, Vernoux T, Dumas R. 2014. Structural basis for oligomerization of auxin transcriptional regulators. Nat Commun. 2014 Apr 7;5:3617.
Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC. 2014. Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32. doi: 10.1073/pnas.1400074111. Epub 2014 Mar 25.
Wang S, Hagen G, Guilfoyle TJ. 2013. ARF-Aux/IAA interactions through domain III/IV are not strictly required for auxin-responsive gene expression. Plant Signal Behav. 2013 Jun;8(6):e24526. Epub 2013 Apr 12.
Guilfoyle TJ, Hagen G. 2012. Getting a grasp on domain III/IV responsible for Auxin Response Factor-IAA protein interactions. Plant Sci. 2012 Jul;190:82-8. Epub 2012 Apr 13. Review.
Research areas: Transcriptional control by auxins in plants; plant RNA polymerases.
How to apply:
Electronic submission is encouraged, e-mail to firstname.lastname@example.org
Applicants should send CV and names of two references to:
Dr. Thomas Guilfoyle
117 Schweitzer Hall
University of Missouri
Columbia, MO 65211