Office: 221 Schweitzer Hall
117 Schweitzer Hall
University of Missouri
Columbia, MO 65211
|MS||Ruhr-Universität||Bochum, Germany||Plant Physiology|
|PhD||Michigan State University||East Lansing, Mich.||PRL-Botany and Plant Pathology|
Sterol requirement in yeast endocytosis (Prof. Howard Riezman, Biozentrum, Univ. of Basel, Switzerland)
Syntaxin phosphorylation in Cf-9/Avr9-immune signaling (Prof. Jonathan Jones. Sainsbury Laboratory, England)
My lab is interested in elucidating the role of membrane trafficking in plant innate immunity. Recent studies indicate that membrane trafficking plays an integral part in plant defenses against invading pathogens because mutations in several plant membrane trafficking components lead to reduced pathogen resistance.
In the model plant Arabidopsis thaliana, innate immunity against bacteria is in part mediated by FLS2, a plasma membrane localized receptor-like kinase. FLS2 perceives a 22 aa-peptide (flg22) derived from flagellin, the building block of the bacterial flagellum. Recognition of flg22 induces defense signaling responses and contributes to plant disease resistance by restricting bacterial invasion. Elicitation with flg22 also leads to ligand-induced endocytosis and degradation of FLS2. However, so far components of the endocytic machinery required for FLS2 internalization and degradation remain largely unknown. My lab is using a combination of protein biochemical, cell biological and molecular biological approaches to identify and characterize proteins functioning in FLS2 endocytosis as well as in other flg22-dependent membrane trafficking events.
Nomination for Outstanding Undergraduate Mentor Award, MU (April 2014)
Nomination for CAFNR-Outstanding Advising Award, MU (Jan 2014)
Nomination for Best Undergraduate Mentor Award, Univ. of Missouri (April 2008)
EMBO Long Term Fellowship (Feb. 2001- Jan. 2003) Sainsbury Laboratory, Norwich, England
Human Frontier Science Program Fellowship (Oct. 1998 - Sep. 2000) Biozentrum, Univ. of Basel, Basel, Switzerland
EMBO Short Term Fellowship (March 1999) Technical Univ. of Graz, Graz, Austria
Fulbright Scholarship (Sep. 1989 - Aug. 1990) MSU-DOE PRL, E. Lansing, USA
Smith JM, Leslie ME, Robinson S, Korasick DA, Zhang T, Backues S, Cornish PV, Koo AJ, Bednarek SY, Heese A. Loss of the Arabidopsis vesicular trafficking protein Dynamin-Related Protein 2B reveals separation of innate immune signaling pathways. PLOS Pathogens 10(12): e1004578. doi:10.1371/journal.ppat.1004578.
Smith JM, Heese A (2014). Rapid bioassay to measure early reactive oxygen species production in Arabidopsis leave tissue in response to living Pseudomonas syringae. Plant Methods. 2014 Feb 26;10(1):6. doi: 10.1186/1746-4811-10-6.
Smith JM, Salamango DJ, Leslie ME, Collins CA, and Heese A Sensitivity to Flg22 Is Modulated by Ligand-Induced Degradation and de Novo Synthesis of the Endogenous Flagellin-Receptor FLAGELLIN-SENSING2. Plant Physiol. 164:440-454 (2014).
Leslie ME, Heese A (2014). A re-elicitation assay to correlate flg22-signaling competency with ligand-induced endocytic degradation of the FLS2 receptor. Methods in Molecular Biology: Plant Endosomes. 1209:149-162; In: Otegui, M (Ed). Humana Press.
Lu D, Lin W, Gao X, Wu S, Cheng C, Avila J, Heese A, Devarenne TP, Ping He P, Shan L. Direct ubiquitination of pattern recognition receptor FLS2 attenuates plant innate immunity. Science. 322: 1439-1442 (2011).
Backues SK, Korasick DA, Heese A, Bednarek SY. The Arabidopsis Dynamin-Related-Protein 2 (DRP2) family is essential for gametophyte development. Plant Cell. 10: 3218-3231 (2010).
Korasick D, McMichael C, Walker KA, Anderson JA, Bednarek SY, Heese A. Novel functions of SCD1 (Stomatal Cytokinesis Defective 1) in plant innate immune responses against bacteria. Journal of Biological Chemistry. 285: 23342-23350 (2010).
Wulff BB, Heese A, Tomlinson-Buhot L, Jones DA, de la Peña M, Jones JD. The major specificity-determining amino acids of the tomato Cf-9 disease resistance protein are at hypervariable solvent exposed positions in the central leucine-rich repeats. Molecular Plant Microbe Interaction. 22:1203-13 (2009).
Shan L, He, P, Li J, Heese A, Peck SP, Nürnberger T, Martin GB, Sheen J. Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host and Microbe. 4, 17-27 (2008).
Heese A, Hann DR, Gimenez-Ibanez S, Jones AME, He K, Li J, Schroeder JI, Peck SC, Rathjen JP. The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc. Natl. Acad. Sci. U.S.A. 104: 12217-12222 (2007).
Heese A, Ludwig AA, Jones JDG. Rapid Phosphorylation of a Syntaxin during the Avr9/Cf-9-Race-Specific Signaling Pathway. Plant Physiology. 138: 2406-2416 (2005).
Heese-Peck A, Pichler H, Zanolari B, Watanabe R, Daum G and Riezman H. Multiple functions of sterols in yeast endocytosis. Molecular Biology of the Cell 13: 2664-80 (2002).
Emter R, Heese-Peck A and Kralli A. ERG6 and PDR5 regulate small lipophilic drug accumulation in yeast cells via distinct mechanisms. FEBS Lett. 19: 521: 57-61 (2002).
D'Hondt K, Heese-Peck A and Riezman H Protein and lipid requirements for endocytosis. Annual Review of Genetics. 34: 255-295 (2000).
Munn AL, Heese-Peck A, Stevenson BJ, Pichler H and Riezman H. Specific sterols required for the internalization step of endocytosis in yeast. Molecular Biology of the Cell. 10: 3943-3957 (1999).