Office: 102 Curtis Hall
Mail: Plant Genetics Research Unit
204 Curtis Hall
University of Missouri
Columbia, MO 65211
|AB||West Virginia University||Morgantown, W.V||Biology|
|MS||West Virginia University||Morgantown, W.V||Plant Physiology|
|PhD||Arizona State University||Tempe, Ariz.||Plant Cell Biology|
Systems biology is a relatively recent term, and can be considered as an amalgam of "functional-omics" data and computational biology. While primarily descriptive as the system is developed, it then evolves to predictive as the system is increasingly defined. The ultimate goal is an integrative model that approaches the in silico organism.
A major focus of systems research in the Miernyk lab is soybean seed development. A descriptive platform has been developed that encompasses eight stages, and defines the periods of cell division/tissue differentiation, cellular specialization, lipid accumulation, protein accumulation, and preparation for quiescence. Total proteins were isolated from each stage and compared by Difference Gel Electrophoresis (DIGE). The patterns of change in protein abundance were analyzed by a combination of model-building, cluster analysis, and multidimensional scaling. Systems cartography is being used to describe the relationships among protein function, expression, localization, and biological interaction. The cartograph is constantly evolving, in all dimensions, as additional data are included.
Miernyk JA. (2016) Getting into mitochondria. Biochemical Journal. 473: 3755-3758.
Gábrišová D, Klubicová K, Danchenko M, Gömöry D, Berezhna VV, Skultety L, Miernyk JA, Rashydov N, Hajduch M. (2016) Do cupins have a function beyond being seed storage proteins? Frontiers Plant Science. 6: 1215.
Miernyk JA, Jett AA, Johnston ML. (2016) Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis. Journal of Proteomics. 130: 56-64.
Rao RSP, Møller IM, Thelen JJ, Miernyk JA. (2015) Convergent signaling pathways- Interaction between methionine oxidation and O-phosphorylation. Cell Stress and Chaperones. 20: 15-21.
Uvackova L, Ondruskova E, Danchenko M, Skultety L, Miernyk JA, Hrubik P, Hajduch M. (2014) establishing a leaf proteome reference map for Ginkgo biloba provides insight into potential ethnobotanical uses. Journal of Agricultural and Food Chemistry. 62: 11547-11556.
Rao RSP, Thelen JJ, Miernyk JA. (2014) In silico analysis of protein Lys-Nɛ-acetylation in plants. Frontiers in Plant Science. 5: 381.
Marty NJ, Teresinski HJ, Hwang YT, Clendening EA, Gidda SG, Sliwinska E, Zhang D, Miernyk JA, Brito GC, Andrews DW, Dyer JM, Mullen RT. (2014) A novel C-terminal dibasic motif mediates the targeting of a subset of tail-anchored proteins to the mitochondrial outer membrane. Frontiers in Plant Science. 5:426.
Rao RSP, Thelen JJ, Miernyk JA. (2014) Is Lys-Nɛ-acetylation the next big thing in post-translational modifications? Trends in Plant Science. 19: 550-553.
Smith-Hammond CL, Hoyos E, Miernyk JA. The pea seedling mithochondrial Nɛ-lysine acetylome. Mitochondrion. 19: 154-165.
Broz AK, Tovar-Méndez A, Mooney BP, Johnston ML, Miernyk JA, Randall DD. A novel regulatory mechanism based upon a dynamic core structure for the mitochondrial pyruvate dehydrogenase complex. Mitochondrion. 19: 144-153.
Flores-Ramirez G, Jankovicova B, Bilkova Z, Ciampor F, Miernyk JA, Skyltety L. (2014) Identification of Coxiella burnetii cell envelope-related proteins using a combined bioinformatics plus proteomics strategy. Proteomics. 14: 1868-1881.
Smith-Hammond CL, Swatek KN, Johnston ML, Thelen JJ, Miernyk JA. (2014) A preliminary description of the developing soybean seed protein-acetylome. Journal of Proteomics. 96: 56-66.
Rao RSP, Xu D, Thelen JJ, Miernyk JA. (2014) Circles within circles: Crosstalk between protein Ser/Thr/Tyr-phosphorylation and Met oxidation. BMC Bioinformatics. 14: S14.
Tovar-Méndez A, Miernyk JA, Hoyos ME, Randall DD. (2014) Functional genomic analysis of Arabidopsis thaliana PP2C clade D. Protoplasma. 251: 265-71.