|BS||University College of Dublin||Dublin, Ireland||Botany|
|PhD||University College of Dublin||Dublin, Ireland||Botany|
I am particularly interested in protocol and techonology development for proteomics and mass spectrometry. We use mass spectrometry for protein identification, protein/peptide quantitation, and mapping sites of post-translational modifications and protein-protein interactions.
Metastable Crystallins: Structure and stabilization (NEI-NIH award 1R01EY023219-01)
Cataract and a host of other diseases result from abnormal interaction of proteins in the cells. The goals of this research are to understand lens protein aggregation in cataract formation and to develop and test lens protein alpha-crystallin-derived peptide as active mini-chaperones capable of suppressing protein aggregation. Understanding the structural changes in mutant proteins associated with cataract can help us learn why certain mutations in lens crystallins lead to cataract. Restoring the lost function in mutant crystallins may control the lens protein aggregation that characterizes cataract formation. Previous studies have shown that peptide chaperones stabilize unfolded proteins. This project will test the ability of peptide chaperones to stabilize and restore activity in mutant and modified lens crystallins. (5/1/2013 to 4/30/2018 - PI/PL: Sharma KK; Co-I: Mooney BP)
Dahal D, Newton KJ, Mooney BP. 2016 Quantitative proteomics of Zea mays hybrids exhibiting different levels of heterosis. J. Proteome Res. 2016 Jun 29. [Epub ahead of print] (2016).
I Taylor, Y Wang, K Seitz, J Baer, S Bennewitz, BP Mooney, JC Walker. Analysis of Phosphorylation of the Receptor-Like Protein Kinase HAESA during Arabidopsis Floral Abscission. PloS One. 11(1) (2016).
T Khare, B Mooney, SE Ridenhour, R Lowery, S Khare, JA Ibdah. Sa1706, a mitochondrial fatty acid oxidation defect causes hepatic steatosis and susceptibility for hepatocellular carcinoma: A proteomics approach. Gastroenterology. 148(4), S-1016 (2015).
M Raju, BP Mooney, KM Thakkar, FJ Giblin, KL Schey, KK Sharma. Role of αA-crystallin-derived αA66-80 peptide in guinea pig lens crystallin aggregation and insolubilization. Experimental Eye Research. 132:151-60 (2015).
AK Broz, A Tovar-Mendez, BP Mooney, ML Johnston, JA Miernyk, DD Randall. A novel reuglatory mechanism based upon a dynamic core structure for the mitochonodrial pyruvate dehydrogenase complex. Mitochondrion. 19:144-153 (2014).
Kannan R, Santhoshkumar P, Mooney BP, Sharma KK. The αA66-80 peptide interacts with soluble α-crystallin and induces its aggregation and precipitation: a contribution to age-related cataract formation. Biochemistry. 52:3638-50 (2013).
Kannan R, Santhoshkumar P, Mooney BP, Sharma KK. Identification of subunit-subunit interaction sites in αA-WT crystallin and mutant αA-G98R crystallin using isotope-labeled cross-linker and mass spectrometry. PLoS One. 8:e65610 (2013).