Stefan G. Sarafianos
Associate Professor of Molecular Microbiology & Immunology
Joint Associate Professor of Biochemistry
E-mail:sarafianoss@missouri.edu
Office: 471D Bond Life Sciences Center
Mail: Christopher S. Bond Life Sciences Center
471D Bond Life Sciences Center
University of Missouri
Columbia, MO 65211
Phone: 573-882-4338
Fax: 573-884-9676
Lab: 573-882-9357
Lab site: http://sarafianoslab.missouri.edu/
Educational background
| Degree | School | Location | Major |
| PhD | Georgetown University | Washington, D.C. | Biochemistry |
| BS | University of Patras | Patras, Greece | Chemistry |
Notable honors and service
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Chancellor's Excellence Chair in Molecular Virology, 2013
Research description
We work towards unraveling the molecular details of how biomedically-relevant enzymes function, how they are inhibited, how they develop drug resistance and towards developing drugs that will treat human disease. In pursuit of these goals we use a combination of conventional and cutting-edge research tools, including protein biochemistry, molecular biology, macromolecular engineering, molecular modeling, X-ray crystallography, enzymology, and high-throughput technologies. Target proteins include HIV-1 reverse transcriptase, SARS RNA-dependent RNA polymerase, SARS 3cl protease, Hepatitis C virus replicase, Hepatitis B reverse transcriptase, and bacterial RNA polymerases.
Selected publications
Tavis JE, Cheng X, Hu Y, Totten M, Cao F, Michailidis E, Aurora R, Meyers MJ, Jacobsen EJ, Parniak MA, Sarafianos SG. The hepatitis B virus ribonuclease H is sensitive to inhibitors of the human immunodeficiency virus ribonuclease H and integrase enzymes. PLoS Pathog. 2013 Jan;9(1):e1003125. doi: 10.1371/journal.ppat.1003125. Epub 2013 Jan 22.
Rai DK, Schafer E, Singh K, McIntosh MA, Sarafianos SG, Rieder E. Repeated exposure to 5D9, an inhibitor of 3D polymerase, effectively limits the replication of foot-and-mouth disease virus in host cells. Antiviral Res. 2013 Apr 8. doi:pii: S0166-3542(13)00085-5. 10.1016/j.antiviral.2013.03.022.
Singh K, Marchand B, Rai DK, Sharma B, Michailidis E, Ryan EM, Matzek KB, Leslie MD, Hagedorn AN, Li Z, Norden PR, Hachiya A, Parniak MA, Xu HT, Wainberg MA, Sarafianos SG. Biochemical mechanism of HIV-1 resistance to rilpivirine. J Biol Chem. 2012 Nov 2;287(45):38110-23. doi: 10.1074/jbc.M112.398180. Epub 2012 Sep 6.
Hachiya A, Marchand B, Kirby KA, Michailidis E, Tu X, Palczewski K, Ong YT, Li Z, Griffin DT, Schuckmann MM, Tanuma J, Oka S, Singh K, Kodama EN, Sarafianos SG. HIV-1 reverse transcriptase (RT) polymorphism 172K suppresses the effect of clinically relevant drug resistance mutations to both nucleoside and non-nucleoside RT inhibitors. J Biol Chem. 2012 Aug 24;287(35):29988-99. doi: 10.1074/jbc.M112.351551. Epub 2012 Jul 2.
Adedeji AO, Singh K, Calcaterra NE, DeDiego ML, Enjuanes L, Weiss S, Sarafianos SG. Severe acute respiratory syndrome coronavirus replication inhibitor that interferes with the nucleic acid unwinding of the viral helicase. Antimicrob Agents Chemother. 2012 Sep;56(9):4718-28. doi: 10.1128/AAC.00957-12. Epub 2012 Jun 25.