Office: 214 Schweitzer Hall
117 Schweitzer Hall
University of Missouri
Columbia, MO 65211
|BA||University of North Carolina||Greensboro, N.C.||Chemistry|
|PhD||Duke University||Durham, N.C.||Biochemistry|
The laboratory looks at metabolism of toxic metals by bacteria that may contribute to the bioremediation of contaminated soils and groundwater.
Have you ever come across a stagnant pool that smelled like rotten eggs when you disturbed it? Or, have you wondered why iron pipes corrode in soil? Both the smell and the corrosion are caused by the sulfate-reducing bacteria that derive energy not from oxygen, but from sulfate, which is reduced to hydrogen sulfide. Do these bacteria only cause problems? Probably not, because we believe they can assist bioremediation, the destruction of toxic contaminants in the environment. Since oxygen kills these bacteria, all work with them must be carried out in the absence of air. Obviously, none but the most committed (stubborn) would work with them.
We are investigating the genetics and metabolism of hydrogen, iron, and sulfate in these bacteria. Just how do they make energy and is hydrogen an important intermediate in their metabolism as well as a substrate? How do they corrode iron? Metallic iron can serve as reductant and the Fe2+ produced is water soluble, until it is irrevocably precipitated by the sulfide ions also produced by the bacteria. Are the bacteria caught in a vicious cycle of removing iron that they need for growth, by the sulfide that is produced during growth? Do they need an iron acquisition system to grow?
The laboratory is also looking at metabolism of toxic metals by these bacteria that may contribute to the bioremediation of contaminated soils and groundwater. These bacteria convert a soluble form of uranium to an insoluble form that precipitates from water. Can we understand how this occurs? Can we increase the rate that this happens? Is the insoluble form used or is it inert forever? Many questions drive the research. Our studies abound with Southerns, cloning, PCRs, transposons, and problems. What we need now is a little intelligent help in understanding these fascinating and challenging organisms.
Smith, S.D., R. Bridou, A. Johs, J.M. Parks, D.A. Elias, R.A. Hurt Jr, S.D. Brown, M. Podar, and J.D. Wall. 2015. Site-directed mutagenesis of HgcA and HgcB reveals amino acid residues important for mercury methylation. Appl Environ Microbiol. 81(9):3205-17. doi: 10.1128/AEM.00217-15.
Ramos, A.R., F. Grein, G.P. Oliveira, S.S. Venceslau, K.L. Keller, J.D. Wall, and I.A. Pereira. 2015. The FlxABCD-HdrABC proteins correspond to a novel NADH dehydrogenase/heterodisulfide reductase widespread in anaerobic bacteria and involved in ethanol metabolism in Desulfovibrio vulgaris Hildenborough. Environ Microbiol. 17(7):2288-305 doi: 10.1111/1462-2920.12689
Christensen, G.A., G.M. Zane, A.E. Kazakov, X. Li, D.A. Rodionov, P.S. Novichkov, I. Dubchak, A.P. Arkin, and J.D. Wall. 2015. Rex (encoded by DVU0916) in Desulfovibrio vulgaris Hildenborough is a repressor of sulfate adenylyl transferase and Is regulated by NADH. J Bacteriol. 197:29-39. doi: 10.1128/JB.02083-14
Korte, H.L., A. Saini, V.V. Trotter, G.P. Butland, A.P. Arkin, and J.D. Wall. 2015. Independence of nitrate and nitrite inhibition of Desulfovibrio vulgaris Hildenborough and use of nitrite as a substrate for growth. Environ Sci Technol 49(2):924-31 PMID:25534748.
Pellerin, A., L. Anderson-Trocmé, L.G. Whyte, G.M. Zane, J.D. Wall, and B.A. Wing. 2015. Sulfur isotope fractionation during the evolutionary adaptation of a sulfate-reducing bacterium. Appl Environ Microbiol. 81(8):2676-89. doi: 10.1128/AEM.03476-14.
Rabus, R., Venceslau, S.S., Wöhlbrand, L., Voordouw, G., Wall, J.D., and Pereira, I.A.C. 2015. A post-genomic view of the ecophysiology, catabolism and biotechnological relevance of sulphate-reducing prokaryotes. R.K. Poole (Series Ed.) Advances in Microbial Physiology, Academic Press an imprint of Elsevier, London, Oxford, and San Diego. Adv Microb Physiol. 66:55-321. PMID: 26210106
Zhou A, Baidoo E, He Z, Mukhopadhyay A, Baumohl JK, Benke P, Joachimiak MP, Xie M, Song R, Arkin AP, Hazen TC, Keasling JD, Wall JD, Stahl DA, Zhou J. Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution. ISME J. 2013 Apr 11. doi: 10.1038/ismej.2013.60.
Parks JM, Johs A, Podar M, Bridou R, Hurt RA Jr, Smith SD, Tomanicek SJ, Qian Y, Brown SD, Brandt CC, Palumbo AV, Smith JC, Wall JD, Elias DA, Liang L. The genetic basis for bacterial mercury methylation. Science. 2013 Mar 15;339(6125):1332-5. doi: 10.1126/science.1230667. Epub 2013 Feb 7.
Rajeev L, Hillesland KL, Zane GM, Zhou A, Joachimiak MP, He Z, Zhou J, Arkin AP, Wall JD, Stahl DA. Deletion of the Desulfovibrio vulgaris carbon monoxide sensor invokes global changes in transcription. J Bacteriol. 2012 Nov;194(21):5783-93. doi: 10.1128/JB.00749-12. Epub 2012 Aug 17.
Ramos AR, Keller KL, Wall JD, Pereira IA. The Membrane QmoABC Complex Interacts Directly with the Dissimilatory Adenosine 5'-Phosphosulfate Reductase in Sulfate Reducing Bacteria. Front Microbiol. 2012;3:137. doi: 10.3389/fmicb.2012.00137. Epub 2012 Apr 23.
Begemann MB, Mormile MR, Sitton OC, Wall JD, Elias DA. A Streamlined Strategy for Biohydrogen Production with Halanaerobium hydrogeniformans, an Alkaliphilic Bacterium. Front Microbiol. 2012;3:93. doi: 10.3389/fmicb.2012.00093. Epub 2012 Mar 21.